Construction, characterization, and use of two Listeria monocytogenes site-specific phage integration vectors.

@article{Lauer2002ConstructionCA,
  title={Construction, characterization, and use of two Listeria monocytogenes site-specific phage integration vectors.},
  author={P. Lauer and Man Yin Nora Chow and M. Loessner and D. Portnoy and R. Calendar},
  journal={Journal of bacteriology},
  year={2002},
  volume={184 15},
  pages={
          4177-86
        }
}
Two site-specific shuttle integration vectors were developed with two different chromosomal bacteriophage integration sites to facilitate strain construction in Listeria monocytogenes. The first vector, pPL1, utilizes the listeriophage U153 integrase and attachment site within the comK gene for chromosomal insertion. pPL1 contains a useful polylinker, can be directly conjugated from Escherichia coli into L. monocytogenes, forms stable, single-copy integrants at a frequency of approximately 10… Expand
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