Construction, characterization, and use of small-insert gene banks of DNA isolated from soil and enrichment cultures for the recovery of novel amidases.

@article{Gabor2004ConstructionCA,
  title={Construction, characterization, and use of small-insert gene banks of DNA isolated from soil and enrichment cultures for the recovery of novel amidases.},
  author={Esther M. Gabor and Erik F. J. de Vries and Dick B. Janssen},
  journal={Environmental microbiology},
  year={2004},
  volume={6 9},
  pages={948-58}
}
To obtain new amidases of biocatalytic relevance, we used microorganisms indigenous to different types of soil and sediment as a source of DNA for the construction of environmental gene banks, following two different strategies. In one case, DNA was isolated from soil without preceding cultivation to preserve a high degree of (phylo)genetic diversity. Alternatively, DNA samples were obtained from enrichment cultures, which is thought to reduce the number of clones required to find a target… CONTINUE READING

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