Construction, calibration, and validation of a simple patch-clamp amplifier for physiology education.

@article{Rouzrokh2009ConstructionCA,
  title={Construction, calibration, and validation of a simple patch-clamp amplifier for physiology education.},
  author={Ali Rouzrokh and Soltan Ahmed Ebrahimi and Massoud Mahmoudian},
  journal={Advances in physiology education},
  year={2009},
  volume={33 2},
  pages={
          121-9
        }
}
A modular patch-clamp amplifier was constructed based on the Strickholm design, which was initially published in 1995. Various parts of the amplifier such as the power supply, input circuit, headstage, feedback circuit, output and nulling circuits were redesigned to use recent software advances and fabricated using the common lithographic printed circuit board fabrication process and commercially available electronic components. The calibration, validation, and regular recording procedures… 
View on PubMed
advan.physiology.org
5 Citations
Background Citations
1

5 Citations

Citation Type

Citation Type

Neuron Emulator Implementations for Patch-Clamp and Longitudinal Electrode Settings
TLDR
The implemented neuron emulator circuits provide the electrical properties of a neuron as seen from a single patch-clamp electrode with a defined action potential (AP) charge output and offers multi-channel output with an adjustable phase delay.
The OpenPicoAmp-100k: an open-source high-performance amplifier for single channel recording in planar lipid bilayers
TLDR
An upgraded version of the previously designed open-source lipid bilayer amplifier, the OpenPicoAmp-100k is proposed, suitable both for the use in introductory courses in biophysics and neurosciences at the undergraduate level and for scientific research.
Implementation of a neuron emulator for patch-clamp settings
TLDR
Measured results from a prototype show that the neuron emulator meets the design specifications and is capable of performing voltage clamp and rate responsive current clamp.
The OpenPicoAmp-100k : an open-source high performance amplifier for single channel recording in planar lipid bilayers
TLDR
An upgraded version of the previously designed open-source lipid bilayer amplifier, the OpenPicoAmp-100k is proposed, suitable both for the use in introductory courses in biophysics and neurosciences at the undergraduate level and for scientific research.
Electronic circuits in patch-clamp system

References

SHOWING 1-10 OF 22 REFERENCES
A hybrid patch clamp amplifier
A single electrode voltage, current- and patch-clamp amplifier with complete stable series resistance compensation
LabPatch, an acquisition and analysis program for patch-clamp electrophysiology.
TLDR
The ability to copy data to other programs running in Windows 95/98, and the ability to keep track of experiments using a database, make LabPatch extremely versatile.
An inexpensive inverted microscope for patch-clamp and other electrophysiological studies at the cellular level
TLDR
Although the instrument was developed for patch-clamp and classical electrophysiological studies, it may be used in other types of investigations where freedom of microtool movement is imperative, such as in microsurgery applications.
An inexpensive inverted microscope for patch-clamp and other electrophysiological studies at the cellular level.
TLDR
Although the instrument was developed for patch-clamp and classical electrophysiological studies, it may be used in other types of investigations where freedom of microtool movement is imperative, such as in microsurgery applications.
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches
Abstract1.The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution,…
Electronic Design of the Patch Clamp
TLDR
This chapter will consider some aspects of the electronic circuitry in greater detail, the main topic being the design of low-noise, wide-bandwidth current-measuring amplifiers.
Supercharging: a method for improving patch-clamp performance.
Patch-clamp analysis : advanced techniques
TLDR
This work presents a meta-analyses of structure-Function Analyses of Single Cells by Combining Patch-Clamp Techniques with RT-PCR and Automated Glass Pipette-based Patch Clamp Techniques, developed by Michael Fejtl and Uwe Czubayko.
Electrical Activity of Individual Neurons: Patch-Clamp Techniques
TLDR
Several recording configurations of the patch-clamp technique enable investigation of macroscopic currents of entire cells as well as elementary single-channel currents in microscopic membrane pieces (patches).
...
1
2
3
...