Construction, binding properties, metabolism, and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49.

@article{Milenic1991ConstructionBP,
  title={Construction, binding properties, metabolism, and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49.},
  author={Diane E. Milenic and T. Yokota and David R. Filpula and Malcolm A. J. Finkelman and Steven Witt Dodd and John F. Wood and Marc Whitlow and Philip J. Snoy and Jeffrey Schlom},
  journal={Cancer research},
  year={1991},
  volume={51 23 Pt 1},
  pages={6363-71}
}
CC49 is a "second generation" monoclonal antibody to B72.3, which reacts with the pancarcinoma antigen TAG-72. CC49 has been shown to efficiently target human colon carcinoma xenografts and is currently being evaluated in both diagnostic and therapeutic clinical trials. We describe here the construction and characterization of a recombinant single-chain Fv (sFv) of CC49. The sFv was shown to be a Mr 27,000 homogeneous entity which could be efficiently radiolabeled with 125I or 131I. Comparative… CONTINUE READING