Construction, affinity maturation, and biological characterization of an anti-tumor-associated glycoprotein-72 humanized antibody.

@article{Yoon2006ConstructionAM,
  title={Construction, affinity maturation, and biological characterization of an anti-tumor-associated glycoprotein-72 humanized antibody.},
  author={Sun Ok Yoon and Tae Sup Lee and Sang Jick Kim and Myung Hee Jang and Young Jun Kang and Jae Hyun Park and Keun-Soo Kim and Hyun Sil Lee and Chun Jeih Ryu and Noreen R. Gonzales and Syed V. S. Kashmiri and Sang Moo Lim and Chang Woon Choi and Hyo Jeong Hong},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 11},
  pages={6985-92}
}
The tumor-associated glycoprotein (TAG)-72 is expressed in the majority of human adenocarcinomas but is rarely expressed in most normal tissues, which makes it a potential target for the diagnosis and therapy of a variety of human cancers. Here we describe the construction, affinity maturation, and biological characterization of an anti-TAG-72 humanized antibody with minimum potential immunogenicity. The humanized antibody was constructed by grafting only the specificity-determining residues… CONTINUE READING