Construct design for efficient, effective and high-throughput gene silencing in plants.

@article{Wesley2001ConstructDF,
  title={Construct design for efficient, effective and high-throughput gene silencing in plants.},
  author={Susan Varsha Wesley and Chris A. Helliwell and Neil A. Smith and M B Wang and Dean T Rouse and Qian Liu and Paul S. Gooding and Sudhir Pratap Singh and Diana Lee Abbott and Peter A. Stoutjesdijk and Simon P Robinson and Andrew P. Gleave and Allan G. Green and Peter M. Waterhouse},
  journal={The Plant journal : for cell and molecular biology},
  year={2001},
  volume={27 6},
  pages={581-90}
}
Post-transcriptional silencing of plant genes using anti-sense or co-suppression constructs usually results in only a modest proportion of silenced individuals. Recent work has demonstrated the potential for constructs encoding self-complementary 'hairpin' RNA (hpRNA) to efficiently silence genes. In this study we examine design rules for efficient gene silencing, in terms of both the proportion of independent transgenic plants showing silencing, and the degree of silencing. Using hpRNA… CONTINUE READING
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