Constraint-based modeling of heterologous pathways: application and experimental demonstration for overproduction of fatty acids in Escherichia coli.

@article{Ip2014ConstraintbasedMO,
  title={Constraint-based modeling of heterologous pathways: application and experimental demonstration for overproduction of fatty acids in Escherichia coli.},
  author={Kuhn Ip and Neil Donoghue and Min Kyung Kim and Desmond S. Lun},
  journal={Biotechnology and bioengineering},
  year={2014},
  volume={111 10},
  pages={
          2056-66
        }
}
Constraint-based modeling has been shown, in many instances, to be useful for metabolic engineering by allowing the prediction of the metabolic phenotype resulting from genetic manipulations. But the basic premise of constraint-based modeling-that of applying constraints to preclude certain behaviors-only makes sense for certain genetic manipulations (such as knockouts and knockdowns). In particular, when genes (such as those associated with a heterologous pathway) are introduced under… CONTINUE READING
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