Constitutively active mutants of rhodopsin

@article{Robinson1992ConstitutivelyAM,
  title={Constitutively active mutants of rhodopsin},
  author={P. Robinson and G. B. Cohen and E. A. Zhukovsky and D. Oprian},
  journal={Neuron},
  year={1992},
  volume={9},
  pages={719-725}
}
  • P. Robinson, G. B. Cohen, +1 author D. Oprian
  • Published 1992
  • Biology, Medicine
  • Neuron
  • Two critical amino acids in the visual pigment rhodopsin are Lys-296, the site of attachment of retinal to the protein through a protonated Schiff base linkage, and Glu-113, the Schiff base counterion. Mutation of Lys-296 or Glu-113 results in constitutive activation of opsin, as assayed by its ability to activate transducin in the absence of added chromophore. We conclude that opsin is constrained to an inactive conformation by a salt bridge between Lys-296 and Glu-113. Recently, one of the… CONTINUE READING
    469 Citations
    Constitutive activity of a UV cone opsin
    • M. Kono
    • Biology, Medicine
    • FEBS letters
    • 2006
    • 16
    • PDF
    G PROTEIN-COUPLED
    • 39
    The C9 methyl group of retinal interacts with glycine-121 in rhodopsin.
    • 57
    • PDF
    The role of Glu181 in the photoactivation of rhodopsin.
    • 83
    The Effects of Amino Acid Replacements of Glycine 121 on Transmembrane Helix 3 of Rhodopsin*
    • 79
    • PDF
    Counterion displacement in the molecular evolution of the rhodopsin family
    • 109

    References

    SHOWING 1-10 OF 41 REFERENCES
    ACID-BASE PROPERTIES OF RHODOPSIN AND OPSIN
    • 86
    • PDF
    Glutamic acid-113 serves as the retinylidene Schiff base counterion in bovine rhodopsin.
    • 547
    • PDF
    Rhodopsin and phototransduction: a model system for G protein‐linked receptors
    • P. Hargrave, J. H. McDowell
    • Biology, Medicine
    • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
    • 1992
    • 303
    Site of Attachment of Retinal in Rhodopsin
    • 306
    The structure of rhodopsin and the rod outer segment disk membrane
    • 221
    The ligand-binding domain of rhodopsin and other G protein-linked receptors
    • D. Oprian
    • Biology, Medicine
    • Journal of bioenergetics and biomembranes
    • 1992
    • 93
    Functional heterogeneity of mutant rhodopsins responsible for autosomal dominant retinitis pigmentosa.
    • 463
    • PDF