Constitutive expression of yeast phospholipid biosynthetic genes by variants of Ino2 activator defective for interaction with Opi1 repressor

@article{Heyken2005ConstitutiveEO,
  title={Constitutive expression of yeast phospholipid biosynthetic genes by variants of Ino2 activator defective for interaction with Opi1 repressor},
  author={Willm-Thomas Heyken and Antje Repenning and Jacqueline Kumme and Hans-Joachim Sch{\"u}ller},
  journal={Molecular Microbiology},
  year={2005},
  volume={56}
}
Regulated expression of structural genes involved in yeast phospholipid biosynthesis is mediated by inositol/choline‐responsive element (ICRE) upstream motifs, bound by the heterodimeric activator complex Ino2 + Ino4. Gene repression occurs in the presence of sufficient inositol and choline, requiring an intact Opi1 repressor which binds to Ino2. For a better understanding of interactions among regulators, we mapped an 18 aa repressor interaction domain (RID, aa 118–135) within Ino2 necessary… Expand
Dimerization of yeast transcription factors Ino2 and Ino4 is regulated by precursors of phospholipid biosynthesis mediated by Opi1 repressor
TLDR
This work systematically investigated the importance of regulatory mechanisms possibly affecting ICRE-dependent gene expression and could show that Ino2-dependent activation of a lexA–Ino4 fusion is affected by inositol and choline. Expand
Opi1 mediates repression of phospholipid biosynthesis by phosphate limitation in the yeast Saccharomyces cerevisiae
TLDR
It is shown that ICRE‐dependent gene activation is repressed not only by an excess of IC but also under conditions of phosphate starvation, and it is postulate that Pho85 positively influences ICRE-dependent gene expression by phosphorylation‐dependent weakening of Opi1 repressor, affecting its functional domains required for promoter recruitment and corepressor interaction. Expand
Mediator subunits and histone methyltransferase Set2 contribute to Ino2-dependent transcriptional activation of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae
TLDR
Analysis of mutants defective for histone methylation and demethylation revealed the importance of the H3 Lys36-specific Set2 methyltransferase for ICRE-dependent gene expression and although defined mediator subunits are critical for gene activation, they could not detect their interaction with Ino2. Expand
Multiple histone deacetylases are recruited by corepressor Sin3 and contribute to gene repression mediated by Opi1 regulator of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae
TLDR
This study systematically investigated the regulatory contribution of subunits of Sin3 complexes and identified Pho23 as important for IC-dependent gene repression and mapped three distinct HDAC interaction domains, designated HID1, HID2 and HID3. Expand
Genomic Analysis of the Opi− Phenotype
TLDR
To better understand the mechanism of Opi1p repression, the viable yeast deletion set was screened to identify Opi− mutants, which included genes with roles in phospholipid biosynthesis, transcription, protein processing/synthesis, and protein trafficking. Expand
Analysis of Opi1p repressor mutants
TLDR
It is shown that novel opi1 mutants that contain missense mutations produce Opi1p product at levels comparable to a wild-type strain, however, these mutants mis-regulate expression of two target genes, INO2-HIS3 and INO1-lacZ, and are also defective in autoregulation. Expand
INO1 induction requires chromatin remodelers Ino80p and Snf2p but not the histone acetylases.
TLDR
It is demonstrated that the recruitment of histone acetylases Gcn5p and Esa1p mainly relied on the presence of transcriptional activator Ino2p during INO1 activation, and the data provide a mechanism for cross talk within transcriptional co-activators during INo1 activation. Expand
Promoter recruitment of corepressors Sin3 and Cyc8 by activator proteins of the yeast Saccharomyces cerevisiae
TLDR
Ch chromatin immunoprecipitation is used to show that Sin3 and Cyc8 can be detected at Opi1 target promoters INO1 and CHO2 under repressing and derepressing conditions and that corepressor binding is effective even in the absence of Opi 1, while Ino2 is absolutely required. Expand
Pleiotropic corepressors Sin3 and Ssn6 interact with repressor Opi1 and negatively regulate transcription of genes required for phospholipid biosynthesis in the yeast Saccharomyces cerevisiae
Repressor protein Opi1 is required to negatively regulate yeast structural genes of phospholipid biosynthesis in the presence of precursor molecules inositol and choline (IC). Opi1 interacts with theExpand
Transcriptional regulation of yeast phospholipid biosynthetic genes.
TLDR
A complete summary of the transcription factors and mechanisms that regulate the phospholipid biosynthetic genes is presented. Expand
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