Constitutive expression of the Trichoderma reesei β-1,4-xylanase gene (xyn2) and the β-1,4-endoglucanase gene (egI) in Aspergillus niger in molasses and defined glucose media

@article{Rose2002ConstitutiveEO,
  title={Constitutive expression of the Trichoderma reesei β-1,4-xylanase gene (xyn2) and the β-1,4-endoglucanase gene (egI) in Aspergillus niger in molasses and defined glucose media},
  author={Shaunita Hellouise Rose and Willem Heber van Zyl},
  journal={Applied Microbiology and Biotechnology},
  year={2002},
  volume={58},
  pages={461-468}
}
The xylanase II (xyn2)- and endoglucanase I (egI)-encoding regions of Trichoderma reesei QM6a were successfully expressed in Aspergillus niger D15 under the transcriptional control of the glyceraldehyde-6-phosphate dehydrogenase (gpd) promoter from A. niger and the glaA terminator of Aspergillus awamori. A stable xyn2 transformant produced β-xylanase activity of 8,000 nkat/ml and 5,000 nkat/ml in shake-flask cultures containing defined or 20% (v/v) molasses medium, respectively. The recombinant… CONTINUE READING
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