Constitutive expression of recombinant proteins in the methylotrophic yeast Hansenula polymorpha using the PMA1 promoter.

@article{Cox2000ConstitutiveEO,
  title={Constitutive expression of recombinant proteins in the methylotrophic yeast Hansenula polymorpha using the PMA1 promoter.},
  author={H T Cox and David Mead and Peter Edwin Sudbery and R M Eland and Ilaria Mannazzu and Louise Evans},
  journal={Yeast},
  year={2000},
  volume={16 13},
  pages={1191-203}
}
The methylotrophic yeast H. polymorpha is a popular system for the expression of recombinant proteins using the strong and regulatable methanol oxidase (MOX) promoter. Here we show that the constitutive PMA1 promoter can programme the expression of two heterologous proteins, glucose oxidase and human serum albumin. A constitutive promoter provides a useful additional facility to the H. polymorpha expression system because it allows a simplified fermentation regime, avoids the use of methanol… CONTINUE READING

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