Constitutive expression of microRNA-150 in mammary epithelium suppresses secretory activation and impairs de novo lipogenesis

@article{Heinz2016ConstitutiveEO,
  title={Constitutive expression of microRNA-150 in mammary epithelium suppresses secretory activation and impairs de novo lipogenesis},
  author={R. Heinz and M. C. Rudolph and Palani Ramanathan and N. Spoelstra and Kiel T. Butterfield and P. Webb and Beatrice L Babbs and Hongwei Gao and Shang Chen and M. Gordon and S. Anderson and M. Neville and Haihua Gu and J. Richer},
  journal={Development},
  year={2016},
  volume={143},
  pages={4236 - 4248}
}
Profiling of RNA from mouse mammary epithelial cells (MECs) isolated on pregnancy day (P)14 and lactation day (L)2 revealed that the majority of differentially expressed microRNA declined precipitously between late pregnancy and lactation. The decline in miR-150, which exhibited the greatest fold-decrease, was verified quantitatively and qualitatively. To test the hypothesis that the decline in miR-150 is crucial for lactation, MEC-specific constitutive miR-150 was achieved by crossing ROSA26… Expand
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