Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis cell cultures

  title={Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis cell cultures},
  author={Aya Ishikawa and Tatsuya Kuma and Hiroyuki Sasaki and Nobuhiro Sasaki and Yoshihiro Ozeki and Nobuyuki Kobayashi and Yoshie Kitamura},
  journal={Plant Cell Reports},
We investigated whether exogenously supplied precursors of bergapten, namely umbelliferone, psoralen and bergaptol, could be utilized to produce bergapten without elicitation in Glehnia littoralis cell suspension cultures. The levels of added psoralen and bergaptol in the medium soon decreased, and this was followed by the detection of bergapten in both culture fluid and cells. Umbelliferone was also incorporated but in this case no bergapten was produced; instead, skimmin, umbelliferone… CONTINUE READING


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