Constitutive expression of a somatic heat-inducible hsp70 gene during amphibian oogenesis.

@article{Billoud1993ConstitutiveEO,
  title={Constitutive expression of a somatic heat-inducible hsp70 gene during amphibian oogenesis.},
  author={B Billoud and Maria-Luz Rodriguez-Martin and L. Berard and Nicolas Moreau and Nicole Angelier},
  journal={Development},
  year={1993},
  volume={119 3},
  pages={921-32}
}
We isolated and characterized a sequence coding for heat-shock protein 70 (HSP70) of the amphibian Pleurodeles waltl. Results from S1 nuclease protection assays led us to conclude that an hsp70 gene, strictly inducible in somatic cells during heat shock, is constitutively active during oogenesis. By quantitative northern and western blot analysis, we showed that both hsp70 mRNA and HSP70-related protein levels increased in oocytes from stage II to stage VI under physiological conditions… CONTINUE READING