We isolated and characterized a sequence coding for heat-shock protein 70 (HSP70) of the amphibian Pleurodeles waltl. Results from S1 nuclease protection assays led us to conclude that an hsp70 gene, strictly inducible in somatic cells during heat shock, is constitutively active during oogenesis. By quantitative northern and western blot analysis, we showed… (More)
Fig. 10. Immunolocalization of HSP70 protein on ovary sections. (A) Previtellogenic (stage I and II) and stage III oocytes. Strong cytoplasmic staining is observed. The nucleus is only faintly stained. (B) Stage IV-V oocyte. HSP70 is localized between yolk platelets. Note that mitochondrial aggregates are particularly fluorescent (arrows). Nucleus immunoreactivity is higher than in (A). (C,D) In stage VI oocyte, staining is either preferentially perinuclear (C, arrows) or nuclear (D). Bar represents 200 µm.