We isolated and characterized a sequence coding for heat-shock protein 70 (HSP70) of the amphibian Pleurodeles waltl. Results from S1 nuclease protection assays led us to conclude that an hsp70 gene, strictly inducible in somatic cells during heat shock, is constitutively active during oogenesis. By quantitative northern and western blot analysis, we showed… (More)
Fig. 6. Northern blot hybridization of ovary constitutive RNA with a [32P]Pw70/3′ cDNA probe. T, 25 µg total RNA; A−, 25 µg poly(A−) RNA; A+, 5 µg poly(A+) RNA; ‘28S’ and ‘18S’ indicate the migration position of rRNAs. A signal is obtained with total and poly(A+) RNA, but not with poly(A−) RNA. The hsp70 mRNA is therefore present in the ovary under normal physiological conditions.