We isolated and characterized a sequence coding for heat-shock protein 70 (HSP70) of the amphibian Pleurodeles waltl. Results from S1 nuclease protection assays led us to conclude that an hsp70 gene, strictly inducible in somatic cells during heat shock, is constitutively active during oogenesis. By quantitative northern and western blot analysis, we showed… (More)
Fig. 2. Strategy for obtaining P. waltl hsp70 sequence. The first step is the cloning of the 3′ moiety of cDNA by immunoscreening of a cDNA library. The second step (PCR) uses sequence information from the cloned sequence, together with sequence homologies in regulation sequences of known hsp70 genes (HSE, i.e. heat-shock responsive element) to amplify the 5′ part from genomic DNA.