We isolated and characterized a sequence coding for heat-shock protein 70 (HSP70) of the amphibian Pleurodeles waltl. Results from S1 nuclease protection assays led us to conclude that an hsp70 gene, strictly inducible in somatic cells during heat shock, is constitutively active during oogenesis. By quantitative northern and western blot analysis, we showed… (More)
Fig. 1. (A) Two-dimensional electrophoresis of a 10000 g supernatant of 10 stage VI oocytes of P. waltl (silver staining according to Morrissey, 1981). Position of the HSP70 protein is denoted by a circle. Molecular mass designations on the left are in 103 Mr. (B) Corresponding immunoblot after incubation with an anti-Plasmodium falciparum HSP70 antibody (mAbH3F18). The antibody recognizes one single polypeptide denoted by a circle (74×103 Mr, pI=5.1).