Constitutive expression of a c-myb cDNA blocks Friend murine erythroleukemia cell differentiation.

@article{Clarke1988ConstitutiveEO,
  title={Constitutive expression of a c-myb cDNA blocks Friend murine erythroleukemia cell differentiation.},
  author={Michael F. Clarke and Jolanta F Kukowska-Latallo and Eric Westin and Melanie Smith and Edward V. Prochownik},
  journal={Molecular and cellular biology},
  year={1988},
  volume={8 2},
  pages={884-92}
}
A full-length human c-myb cDNA clone has been isolated from a CCRF-CEM leukemia cell cDNA library. The plasmid vector contains simian virus 40-derived promotor, splice, and polyadenylation sequences as well as a transcription unit for a dihydrofolate reductase cDNA. We have introduced this construct into Friend erythroleukemia (F-MEL) cells and have isolated a number of clones which contain intact and transcriptionally active human c-myb sequences. F-MEL clones expressing the highest levels of… CONTINUE READING