Constitutive and inducible green fluorescent protein expression in Bartonella henselae.

@article{Lee1998ConstitutiveAI,
  title={Constitutive and inducible green fluorescent protein expression in Bartonella henselae.},
  author={Aimee Lee and Stanley Falkow},
  journal={Infection and immunity},
  year={1998},
  volume={66 8},
  pages={3964-7}
}
The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing bacteria were separated from uninfected cells with a fluorescence activated cell sorter. Promoter fusions of B. henselae chromosomal DNA to gfp were examined by flow cytometry, and a B. henselae groEL promoter fusion which induced expression at 37 degreesC was isolated. 
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