Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection.

@article{Hovig1991ConstantDG,
  title={Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection.},
  author={Eivind Hovig and Birgitte Smith-S\orensen and A Br\ogger and A. L. B\orresen},
  journal={Mutation research},
  year={1991},
  volume={262 1},
  pages={63-71}
}
Denaturing gradient gel electrophoresis (DGGE) is increasingly being utilized in mutational detection, both in characterization of variations in genomic DNA and in the generation of mutational spectra after in vitro and in vivo mutagenesis. The basis for this electrophoretic separation technique is strand dissociation of DNA fragments in discrete, sequence-dependent melting domains followed by an abrupt decrease in mobility. We have modified the DGGE by using constant denaturant gels… CONTINUE READING

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