Considerations on bacterial nucleoids

  title={Considerations on bacterial nucleoids},
  author={Luc{\'i}a Feijoo-Siota and Jos{\'e} Luis R Rama and Angeles Sanchez-Perez and Tom{\'a}s G. Villa},
  journal={Applied Microbiology and Biotechnology},
The classic genome organization of the bacterial chromosome is normally envisaged with all its genetic markers linked, thus forming a closed genetic circle of duplex stranded DNA (dsDNA) and several proteins in what it is called as “the bacterial nucleoid.” This structure may be more or less corrugated depending on the physiological state of the bacterium (i.e., resting state or active growth) and is not surrounded by a double membrane as in eukayotic cells. The universality of the closed… 

On the maverick Planctomycetes

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Dinoroseobacter shibae Outer Membrane Vesicles Are Enriched for the Chromosome Dimer Resolution Site dif

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It is argued that the spatial organization of genome plays a fundamental role in its own regulation, in which activation of functionally relevant genes is coupled to and coordinated with the establishment of extended chromosomal domains of coherent transcription.

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This article reviews the history of the study of PVC bacteria, with a particular focus on the misinterpretations that emerged early in the field and their resolution, and argues that the future of this growing community is looking bright.

Étude de l’organisation et de la ségrégation du chromosome de Pseudomonas aeruginosa.

Au moment de la division cellulaire, l’ADN contenu dans les chromosomes doit etre transmis de la cellule mere a chacune des cellules filles dans chacun des cellule c’est a dire apres that les chromosomes soient completement repliques.

Introductory Chapter

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Advances are now being made in understanding how chromosome organization is achieved in multi-genomic hyphal compartments, in defining the functional and regulatory interplay between different architectural elements, and in appreciating the transcriptional control exerted by these 'structural' proteins.

Commentary: Manifold Routes to a Nucleus



Organization of DNA in a bacterial nucleoid

The results suggest that DNA is packaged in the bacterial nucleoid in a non-random way that facilitates interaction of the DNA binding factors with regulatory regions of the genome.

The bacterial nucleoid visualized by fluorescence microscopy of cells lysed within agarose: comparison of Escherichia coli and spirochetes of the genus Borrelia

The nucleoids of Escherichia coli and the spirochetes Bor Relia burgdorferi and Borrelia hermsii, agents of Lyme disease and relapsing fever, were examined by epifluorescence microscopy of bacterial cells embedded in agarose and lysed in situ with detergent and protease to analyze the structure of the bacterial nucleoid.

Escherichia coli with a linear genome

There were no appreciable differences between cells with linear or circular genomes in growth rates, cell and nucleoid morphologies, genome‐wide gene expression (with a few exceptions), and DNA gyrase‐ and topoisomerase IV‐dependent growth.

Building bridges within the bacterial chromosome.

Function of Nucleoid-Associated Proteins in Chromosome Structuring and Transcriptional Regulation

  • C. Dorman
  • Biology
    Journal of Molecular Microbiology and Biotechnology
  • 2014
The dynamic composition of the population of nucleoid-associated proteins in model bacteria such as Escherichia coli and Salmonella enterica links nucleoid structure and the global regulation of gene expression, enhancing microbial competitive fitness and survival in complex environments.

Bacterial chromosome organization and segregation.

Drawing on studies of many different organisms, the emerging picture of how bacterial chromosomes are structured at multiple length scales is reviewed, highlighting the functions of various DNA-binding proteins and the impact of physical forces.

The bacterial ‘mitochondrium’

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Effects of nucleoid-associated proteins on bacterial chromosome structure and gene expression.