Conserved nucleotide sequences at the 5' end of T cell receptor variable genes facilitate polymerase chain reaction amplification.

@article{Broeren1991ConservedNS,
  title={Conserved nucleotide sequences at the 5' end of T cell receptor variable genes facilitate polymerase chain reaction amplification.},
  author={Chris P. M. Broeren and Georges M G M Verjans and Willem van Eden and Johannes G Kusters and Johannes A Lenstra and Ton Logtenberg},
  journal={European journal of immunology},
  year={1991},
  volume={21 3},
  pages={569-75}
}
Alignment of all available nucleotide sequences of mouse and rat alpha/beta T cell receptor (TcR) variable (V) regions revealed the presence of relatively conserved sequences at the 5' end of the V gene segments. Based on these conserved sequences, degenerate primers were developed for use in the polymerase chain reaction (PCR). The degenerate primers developed on the basis of the conserved sequences at the 5' end of rat and mouse V gene segments are expected to enable the amplification of all… CONTINUE READING

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