Conservation of the follicular population in irradiated rats by the cryopreservation and orthotopic autografting of ovarian tissue.

@article{Aubard1998ConservationOT,
  title={Conservation of the follicular population in irradiated rats by the cryopreservation and orthotopic autografting of ovarian tissue.},
  author={Yves Aubard and Harry Newton and G. J. Scheffer and Roger G. Gosden},
  journal={European journal of obstetrics, gynecology, and reproductive biology},
  year={1998},
  volume={79 1},
  pages={83-7}
}
AIM To preserve the follicular population in rats prior to abdominal irradiation by the orthotopic autografting of ovarian cortical slices (frozen/thawed) in one of two cryoprotective agents (CPAs). The proportion of follicular survival will be quantified at autopsy and a comparison will be made of the two CPAs. METHOD Thirty Wistar rats, aged 38-39 days, were unilaterally ovariectomised. The ovaries of twenty animals were slow-cooled in either 1.5 M dimethylsulphoxide (DMSO; group B) or 1.5… CONTINUE READING

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