Conservation of human vs. feline genome organization revealed by reciprocal chromosome painting.

@article{Wienberg1997ConservationOH,
  title={Conservation of human vs. feline genome organization revealed by reciprocal chromosome painting.},
  author={Johannes Wienberg and Roscoe Stanyon and William G. Nash and Patricia C M O'brien and Fengtang Yang and Stephen J. O'Brien and M. A. Ferguson-Smith},
  journal={Cytogenetics and cell genetics},
  year={1997},
  volume={77 3-4},
  pages={211-7}
}
We employed fluorescence in situ hybridization (FISH) with probes established by flow sorting metaphase chromosomes of the domestic cat (Felis cattus, 2n = 38) to "paint" homologous segments on human chromosomes and, reciprocally, using human chromosome paints on feline metaphase preparations. The results revealed, by direct microscopic observation, widespread conservation of genome organization between the two mammalian orders and confirmed 90% of the homologous genes mapped to both species… CONTINUE READING