Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii.

@article{Meyer2009ConsensusMS,
  title={Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii.},
  author={Wieland Meyer and David M. Aanensen and Teun Boekhout and Massimo Cogliati and Mara R. Diaz and Maria Carmela Esposto and Matthew Fisher and Félix Gilgado and Ferry Hagen and Sirada Kaocharoen and Anastasia P. Litvintseva and Thomas G. Mitchell and Sitali P. Simwami and Luciana Trilles and Maria Anna Viviani and June Kwon-Chung},
  journal={Medical mycology},
  year={2009},
  volume={47 6},
  pages={
          561-70
        }
}
This communication describes the consensus multi-locus typing scheme established by the Cryptococcal Working Group I (Genotyping of Cryptococcus neoformans and C. gattii) of the International Society for Human and Animal Mycology (ISHAM) using seven unlinked genetic loci for global strain genotyping. These genetic loci include the housekeeping genes CAP59,GPD1, LAC1, PLB1, SOD1, URA5 and the IGS1 region. Allele and sequence type information are accessible at http://www.mlst.net/ . 

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References

SHOWING 1-10 OF 55 REFERENCES
Development of polymorphic microsatellite markers for Cryptococcus neoformans
TLDR
Eleven microsatellite loci that were developed from the published genomes of C. neoformans var.
Characterization of Cryptococcus neoformans by random DNA amplification
TLDR
Random amplified polymorphic DNA (RAPD) was optimized and used to distinguish between the varieties and serotypes of Cryptococcus neofirmans and revealed further differentiation within each serotype.
Unique hybrids between the fungal pathogens Cryptococcus neoformans and Cryptococcus gattii.
TLDR
This study is the first to describe naturally occurring hybrids between C. neoformans and C. gattii, which are believed to be monokaryotic, and diploid or aneuploid.
Multilocus Sequence Typing Reveals Three Genetic Subpopulations of Cryptococcus neoformans var. grubii (Serotype A), Including a Unique Population in Botswana
We applied multilocus sequence typing (MLST) to investigate the population structure and mode of reproduction of Cryptococcus neoformans var. grubii (serotype A). This MLST system utilizes 12
Use of a dispersed repetitive DNA element to distinguish clinical isolates of Cryptococcus neoformans
TLDR
Hybridization of CNRE-1 to restriction digests of genomic DNA confirmed that there are multiple copies of this element and that restriction fragment length polymorphisms are present in strains from different serotypes of C. neoformans.
Cryptococcus neoformans var.grubii: Separate Varietal Status for Cryptococcus neoformans Serotype A Isolates
TLDR
It is proposed to recognize genotypic distinctions between the URA5 sequences and DNA fingerprinting patterns, as well as previously reported phenotypic differences, by restricting C. neoformans to isolates which are serotype D and describing a new variety, C. grubii, for serotype A isolates.
Multilocus microsatellite typing for Cryptococcus neoformans var. grubii.
TLDR
The three polymorphic microsatellites are useful markers for strain genotyping, population genetic analysis, epidemiological studies, and may be helpful for the diagnosis of cryptococcosis due to C. neoformans.
Molecular typing of global isolates of Cryptococcus neoformans var. neoformans by polymerase chain reaction fingerprinting and randomly amplified polymorphic DNA — a pilot study to standardize techniques on which to base a detailed epidemiological survey
TLDR
All US isolates could be differentiated by a unique, strain‐specific PCR fingerprint or RAPD pattern in contrast to most of the non‐US isolates, which showed a substantially higher degree of genetic homogeneity, with some clonality, in different parts of the world.
Simultaneous identification of molecular and mating types within the Cryptococcus species complex by PCR-RFLP analysis.
TLDR
Two PCR-RFLP analyses, based on the CAP1 and GEF1 genes, were developed for simultaneous identification of the molecular and mating types of isolates of the Cryptococcus species complex, and pattern analysis of the AD hybrids revealed that the serotype A MATa allele in strains of AaDalpha derived from genotype VNB, whereas the serotypes A MATalpha and AalphaDa derived from molecular type VNI.
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