Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output.

  title={Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output.},
  author={Andreas M Loening and Timothy David Fenn and Anna M Wu and Sanjiv Sam Gambhir},
  journal={Protein engineering, design & selection : PEDS},
  volume={19 9},
Luciferases, which have seen expansive employment as reporter genes in biological research, could also be used in applications where the protein itself is conjugated to ligands to create probes that are appropriate for use in small animal imaging. As the bioluminescence activity of commonly used luciferases is too labile in serum to permit this application, specific mutations of Renilla luciferase, selected using a consensus sequence driven strategy, were screened for their ability to confer… CONTINUE READING
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