Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input.

@article{Aerts2013ConsensusEO,
  title={Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input.},
  author={D. J. Aerts and Tom F Verhaeghe and Henk-Jan Joosten and Gert Vriend and Wim K. Soetaert and Tom Desmet},
  journal={Biotechnology and bioengineering},
  year={2013},
  volume={110 10},
  pages={2563-72}
}
Consensus engineering, which is replacing amino acids by the most frequently occurring one at their positions in a multiple sequence alignment (MSA), is a known strategy to increase the stability of a protein. The application of this concept to the entire sequence of an enzyme, however, has been tried only a few times mainly because of the problems… CONTINUE READING