Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinase.

@article{Stephan2004ConsecutiveGD,
  title={Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinase.},
  author={Joachim Stephan and Volker Stemmer and Michael Niederweis},
  journal={Gene},
  year={2004},
  volume={343 1},
  pages={181-90}
}
Mycobacteria contain a large number of redundant genes whose functions are difficult to analyze in mutants, because there are only two efficient resistance markers available for allelic exchange experiments. We have established a system based on the Flp recombinase of the yeast Saccharomyces cerevisiae for use in the nonpathogenic model organism Mycobacterium smegmatis. This system consists of a hygromycin resistance cassette flanked by two Flp recognition targets (FRT) in direct orientation… CONTINUE READING

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