Consecutive gene deletions in Aspergillus nidulans: application of the Cre/loxP system

@article{Forment2006ConsecutiveGD,
  title={Consecutive gene deletions in Aspergillus nidulans: application of the Cre/loxP system},
  author={Josep V Forment and Daniel Ram{\'o}n and Andrew P Maccabe},
  journal={Current Genetics},
  year={2006},
  volume={50},
  pages={217-224}
}
The ability to perform multiple gene deletions is an important tool for conducting functional genomics. We report the development of a sequential gene deletion protocol for the filamentous fungus Aspergillus nidulans using the Cre/loxP recombinase system of bacteriophage P1. A recyclable genetic marker has been constructed by incorporating loxP direct repeats either side of the Neurospora crassa pyr-4 gene (encodes orotidine 5′-monophosphate decarboxylase) which is able to complement the A… CONTINUE READING

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