Connexin Composition in Apposed Gap Junction Hemiplaques Revealed by Matched Double-Replica Freeze-Fracture Replica Immunogold Labeling

@inproceedings{Rash2012ConnexinCI,
  title={Connexin Composition in Apposed Gap Junction Hemiplaques Revealed by Matched Double-Replica Freeze-Fracture Replica Immunogold Labeling},
  author={John E Rash and Naomi Kamasawa and Kimberly G. V. Davidson and Thomas Yasumura and Alberto E Pereda and J. I. Nagy},
  booktitle={The Journal of Membrane Biology},
  year={2012}
}
Despite the combination of light-microscopic immunocytochemistry, histochemical mRNA detection techniques and protein reporter systems, progress in identifying the protein composition of neuronal versus glial gap junctions, determination of the differential localization of their constituent connexin proteins in two apposing membranes and understanding human neurological diseases caused by connexin mutations has been problematic due to ambiguities introduced in the cellular and subcellular… CONTINUE READING