Connexin‐specific cell‐to‐cell transfer of short interfering RNA by gap junctions

  title={Connexin‐specific cell‐to‐cell transfer of short interfering RNA by gap junctions},
  author={V. Valiunas and Y. Y. Polosina and H. Miller and I. Potapova and L. Valiuniene and S. Doronin and R. Mathias and R. Robinson and M. Rosen and I. Cohen and P. Brink},
  journal={The Journal of Physiology},
  • V. Valiunas, Y. Y. Polosina, +8 authors P. Brink
  • Published 2005
  • Biology, Medicine
  • The Journal of Physiology
  • The purpose of this study was to determine whether oligonucleotides the size of siRNA are permeable to gap junctions and whether a specific siRNA for DNA polymerase β (pol β) can move from one cell to another via gap junctions, thus allowing one cell to inhibit gene expression in another cell directly. To test this hypothesis, fluorescently labelled oligonucleotides (morpholinos) 12, 16 and 24 nucleotides in length were synthesized and introduced into one cell of a pair using a patch pipette… CONTINUE READING
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