Connecting nitrogenase intermediates with the kinetic scheme for N2 reduction by a relaxation protocol and identification of the N2 binding state.

@article{Lukoyanov2007ConnectingNI,
  title={Connecting nitrogenase intermediates with the kinetic scheme for N2 reduction by a relaxation protocol and identification of the N2 binding state.},
  author={Dmitriy A. Lukoyanov and Brett M Barney and Dennis R Dean and Lance C Seefeldt and Brian M Hoffman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2007},
  volume={104 5},
  pages={
          1451-5
        }
}
A major obstacle to understanding the reduction of N2 to NH3 by nitrogenase has been the impossibility of synchronizing electron delivery to the MoFe protein for generation of specific enzymatic intermediates. When an intermediate is trapped without synchronous electron delivery, the number of electrons, n, it has accumulated is unknown. Consequently, the intermediate is untethered from kinetic schemes for reduction, which are indexed by n. We show that a trapped intermediate itself provides a… CONTINUE READING

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