Conjugative plasmid mediated inducible nickel resistance in Hafnia alvei 5-5.

  title={Conjugative plasmid mediated inducible nickel resistance in Hafnia alvei 5-5.},
  author={Jeong Eun Park and Kho Eun Young and H. -J. Schlegel and Ho Gun Rhie and Ho Sa Lee},
  journal={International microbiology : the official journal of the Spanish Society for Microbiology},
  volume={6 1},
Hafnia alvei 5-5, isolated from a soil-litter mixture underneath the canopy of the nickel-hyperaccumulating tree Sebertia acuminata (Sapotaceae) in New Caledonia, was found to be resistant to 30 mM Ni(2+) or 2 mM Co(2+). The 70-kb plasmid, pEJH 501, was transferred by conjugation to Escherichia coli, Serratia marcescens, and Klebsiella oxytoca. Transconjugant strains expressed inducible nickel resistance to between 5 and 17 mM Ni(2+), and cobalt resistance to 2 mM Co(2+). A 4.8-kb Sal- EcoRI… CONTINUE READING
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