Conjugation of p-aminophenyl glycosides with squaric acid diester to a carrier protein and the use of neoglycoprotein in the histochemical detection of lectins.

Abstract

The coupling of p-aminophenyl 2-acetamido-2-deoxy-3-O-beta-D- galactopyranosyl-beta-D-galactopyranoside (gal-beta 1,3-galNAc) to bovine serum albumin (BSA) was achieved by using 1,2-diethoxycyclobutene-3,4-dione (squaric acid diester) as a new coupling reagent. Two selective consequential steps afforded the desired neoglycoprotein: reaction of the p… (More)

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@article{Tietze1991ConjugationOP, title={Conjugation of p-aminophenyl glycosides with squaric acid diester to a carrier protein and the use of neoglycoprotein in the histochemical detection of lectins.}, author={Lutz F Tietze and C. D. Schroeter and Sigrun Gabius and Ute Brinck and Ada Goerlach-Graw and Hans Joachim Gabius}, journal={Bioconjugate chemistry}, year={1991}, volume={2 3}, pages={148-53} }