Conjugation of antibodies to fluorescein or rhodamine.

@article{Wisdom2005ConjugationOA,
  title={Conjugation of antibodies to fluorescein or rhodamine.},
  author={Gregory Wisdom},
  journal={Methods in molecular biology},
  year={2005},
  volume={295},
  pages={131-4}
}
The isothiocyanate derivative of the fluorophore, fluorescein or rhodamine, is coupled to the amino groups of IgG antibody in a one-step procedure and excess label is removed by gel filtration.