Conjugation of 5-azido-3-oxapentyl glycosides with thiolated proteins through the use of thiophilic derivatives

  title={Conjugation of 5-azido-3-oxapentyl glycosides with thiolated proteins through the use of thiophilic derivatives},
  author={Violeta Fern{\'a}ndez-Santana and Raul Gonzalez-Lio and Jorge Sarracent-P{\'e}rez and vicente Verez-Bencomo},
  journal={Glycoconjugate Journal},
5-Azido-3-oxa-pentyl beta-D-galactopyranoside was prepared from diethylene glycol monochlorohydrin and used as a model of oligosaccharide hapten. After deprotection, a series of amides bearing thiophilic groups had been obtained through the terminal amino function and essayed in coupling reactions with thiolated BSA. Also several Lewis human blood group oligosaccharides had been conjugated with thiolated BSA demonstrating the usefulness of the methodology. 
Synthesis of Neoglycolipid Analogues of the Oligosaccharide Portion of Ganglioside GM3
Two neoglicolipids containing as a lipid, a racemic glycerol unit substituted by two aliphatic octadecyl ether chains are synthesized, revealing the molecular specificity of the antimelanoma response induce by GM3 included in proteoliposome preparation.
3'-sialyllactose neoglycoconjugates are not able to provide an immunological response against N-acetyl GM3 ganglioside in mice and chickens
Five different neoglycoconjugates containing 3'-sialyllactose or related structures linked covalently to Human Serum Albumin were prepared as immunogens in order to elicit an immune response crossreacting with GM3, and induced a moderate to strong immune response against the oligosaccharide.
The potential of dextran-based glycoconjugates for development of Helicobacter pylori vaccine
G glycoconjugates based on dextrans produced by lactic acid bacteria Leuconostoc mesenteroides B512F and consisting of linear α(1-6)-glucan chains with limited branching provide evidence that dextran-based conjugates may offer a simplified approach to the development of carbohydrate-based vaccines against H. pylori.
Fullerene-capped copolymers for bulk heterojunctions: device stability and efficiency improvements
A fullerene end-capped polymer-compatibilizer based on poly(3-hexylthiophene) (P3HT) was synthesized and demonstrated to have a remarkable effect on both the stability and efficiency of devices made
Synthetic Teichoic Acid Conjugate Vaccine against Nosocomial Gram-Positive Bacteria
In all animal models, a statistically significant reduction of colony counts was observed indicating that the novel synthetic LTA-fragment conjugate is a promising vaccine candidate for active or passive immunotherapy against E. faecalis and other Gram-positive bacteria.
Synthesis of fragments of the capsular polysaccharide of Neisseria meningitidis (serogroup A) suitable for bioconjugation
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A Synthetic Conjugate Polysaccharide Vaccine Against Haemophilus influenzae Type b
The large-scale synthesis, pharmaceutical development, and clinical evaluation of a conjugate vaccine composed of a synthetic capsular polysaccharide antigen of Haemophilus influenzae type b (Hib) demonstrate that access to synthetic complex carbohydrate–based vaccines is feasible and provides a basis for further development of similar approaches for other human pathogens.
Conjugues de proteine serique de composes de modulation du canal ionique, utilisations de ces derniers
La presente invention concerne des conjugues de proteine serique de composes de modulation du canal ionique, tels que par exemple, des conjugues de proteine serique du compose de modulation du canal
Approche multivalente des interactions saccharides - lectines : synthèse de glycoclusters et analyse de la reconnaissance biomoléculaire
L'interaction non-covalente entre un ligand et un recepteur selon un modele cle-serrure constitue une des bases essentielles de tout systeme biologique. La presence de multiples cles et serrures sur


Neoglycoconjugates: Preparation and Applications pp 53—143
  • 1994
Carbohydr Res 150: 199—212
  • J Carbohydr Chem
  • 1986