Conformational transition of DNA bound to Hfq probed by infrared spectroscopy.

  title={Conformational transition of DNA bound to Hfq probed by infrared spectroscopy.},
  author={Fr{\'e}d{\'e}ric Geinguenaud and Vania Calandrini and Jose Teixeira and Claudine Mayer and Jean Liquier and Christophe Lavelle and V{\'e}ronique Arluison},
  journal={Physical chemistry chemical physics : PCCP},
  volume={13 3},
Hfq is a bacterial protein involved in RNA metabolism. Besides this, Hfq's role in DNA restructuring has also been suggested. Since this mechanism remains unclear, we examined the DNA conformation upon Hfq binding by combining vibrational spectroscopy and neutron scattering. Our analysis reveals that Hfq, which preferentially interacts with deoxyadenosine rich sequences, induces partial opening of dA-dT sequences accompanied by sugar repuckering of the dA strand and hence results in a… CONTINUE READING

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