Conformational stability of Cys45-alkylated and hydrogen peroxide-oxidised glutathione S-transferase.

@article{SluisCremer1995ConformationalSO,
  title={Conformational stability of Cys45-alkylated and hydrogen peroxide-oxidised glutathione S-transferase.},
  author={N Sluis-Cremer and Heini W Dirr},
  journal={FEBS letters},
  year={1995},
  volume={371 2},
  pages={94-8}
}
A highly reactive cysteine residue in class pi glutathione S-transferases enhances their susceptibility to chemical alkylation and oxidative stress. Alkylation of the reactive Cys45 in the porcine class pi enzyme (pGSTP1-1) with either N-iodoacetyl-N'-(5-sulpho-1-naphthyl)ethylenediamine or iodoacetamide results in a loss of enzyme activity and glutathione… CONTINUE READING