Conformational stability and activity of ribonuclease T1 with zero, one, and two intact disulfide bonds.

@article{Pace1988ConformationalSA,
  title={Conformational stability and activity of ribonuclease T1 with zero, one, and two intact disulfide bonds.},
  author={Cynthia Pace and Gerald R. Grimsley and John Arthur Thomson and Barbara J Barnett},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 24},
  pages={
          11820-5
        }
}
Ribonuclease T1 has two disulfide bonds linking cysteine residues 2-10 and 6-103. We have prepared a derivative of ribonuclease T1 in which one disulfide bond is broken and the cysteine residues carboxymethylated, (2-10)-RCM-T1, and three derivatives in which both disulfides are broken and the cysteine residues reduced, R-T1, carboxamidomethylated, RCAM-T1, or carboxymethylated, RCM-T1. The RNA hydrolyzing activity of these proteins has been measured, and urea and thermal denaturation studies… CONTINUE READING

Similar Papers

Citations

Publications citing this paper.
SHOWING 1-10 OF 105 CITATIONS, ESTIMATED 38% COVERAGE

Study and design of stability in GH5 cellulases.

  • Biotechnology and bioengineering
  • 2012
VIEW 4 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Disulfide bond effects on protein stability: designed variants of Cucurbita maxima trypsin inhibitor-V.

  • Protein science : a publication of the Protein Society
  • 2001
VIEW 9 EXCERPTS
CITES BACKGROUND & RESULTS
HIGHLY INFLUENCED

Disulfide bonds and the stability of globular proteins.

  • Protein science : a publication of the Protein Society
  • 1993
VIEW 9 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

The effects of disulfide bonds on the denatured state of barnase.

  • Protein science : a publication of the Protein Society
  • 2000
VIEW 4 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

Construction and characterization of protein libraries composed of secondary structure modules.

  • Protein science : a publication of the Protein Society
  • 2002
VIEW 2 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

1992
2019

CITATION STATISTICS

  • 7 Highly Influenced Citations