Conformational equilibria of light-activated rhodopsin in nanodiscs.

  title={Conformational equilibria of light-activated rhodopsin in nanodiscs.},
  author={Ned Van Eps and Lydia N. Caro and Takefumi Morizumi and Ana Karin Kusnetzow and Michal Szczepek and Klaus Peter Hofmann and Timothy H. Bayburt and Stephen G Sligar and Oliver P Ernst and Wayne L. Hubbell},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={114 16},
Conformational equilibria of G-protein-coupled receptors (GPCRs) are intimately involved in intracellular signaling. Here conformational substates of the GPCR rhodopsin are investigated in micelles of dodecyl maltoside (DDM) and in phospholipid nanodiscs by monitoring the spatial positions of transmembrane helices 6 and 7 at the cytoplasmic surface using site-directed spin labeling and double electron-electron resonance spectroscopy. The photoactivated receptor in DDM is dominated by one… CONTINUE READING