Conformational dynamics of loops L11 and L12 of kinesin as revealed by spin-labeling EPR.

@article{Yamada2007ConformationalDO,
  title={Conformational dynamics of loops L11 and L12 of kinesin as revealed by spin-labeling EPR.},
  author={Masafumi D Yamada and Shinsaku Maruta and Satoshi Yasuda and Kazunori Kondo and Hidekatsu Maeda and Toshiaki Arata},
  journal={Biochemical and biophysical research communications},
  year={2007},
  volume={364 3},
  pages={620-6}
}
The EPR spectra of the spin labels attached to loops L11 and L12 of kinesin were resolved into slow (rotational correlation time, tau=10-45 ns) and fast (tau=2 ns) components. The fraction of the slow component increased considerably when kinesin was complexed with a microtubule (MT). On MT binding and in the presence of nucleotides ADP and AMPPNP, the spin labels on L11, particularly at A252C and L249C, significantly decreased the fraction of the slow component. Moreover, dipolar EPR detected… CONTINUE READING