Conformational changes in the intestinal brush border sodium-glucose cotransporter labeled with fluorescein isothiocyanate.

@article{Peerce1984ConformationalCI,
  title={Conformational changes in the intestinal brush border sodium-glucose cotransporter labeled with fluorescein isothiocyanate.},
  author={Brian E. Peerce and Ernest M Wright},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 7},
  pages={2223-6}
}
Fluorescein isothiocyanate (FITC) was used to label the rabbit intestinal brush border Na+-glucose carrier, identify the carrier protein on sodium dodecyl sulfate/polyacrylamide gel electrophoresis, and monitor the effect of ions and substrates on fluorescence quenching. Enriched brush border preparations were employed to study both glucose transport and FITC binding. FITC and a nonfluorescent analog (phenyl isothiocyanate, PITC) both inhibited Na+-dependent D-glucose transport irreversibly… CONTINUE READING