A fluorophore, 9-anthroyl (AN) group, was covalently incorporated into the 23-kDa NH2-terminal peptide segment of myosin subfragment-1 (S-1) (Hiratsuka, T. (1989) J. Biol. Chem. 264, 18188-18194). The fluorescent S-1 derivative (AN-S-1) was utilized to detect conformational changes in the 23-kDa segment associated with ATP hydrolysis of S-1. The extrinsic… (More)
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