Conformational changes and inactivation of calf intestinal alkaline phosphatase in trifluoroethanol solutions.

@article{Zhang2000ConformationalCA,
  title={Conformational changes and inactivation of calf intestinal alkaline phosphatase in trifluoroethanol solutions.},
  author={Ying-xia Zhang and Ya-qin Zhu and Hai Meng Zhou},
  journal={The international journal of biochemistry & cell biology},
  year={2000},
  volume={32 8},
  pages={887-94}
}
The changes in activity and unfolding of calf intestinal alkaline phosphatase (CIP) during denaturation in different concentrations of trifluoroethanol (TFE) have been investigated by far-ultraviolet circular dichroism and fluorescence emission spectra. Unfolding and activation rate constants were measured and compared, the activation and inactivation… CONTINUE READING