Conformational and functional significance of residue proline 17 in chicken muscle adenylate kinase

  title={Conformational and functional significance of residue proline 17 in chicken muscle adenylate kinase},
  author={Xiangrong Sheng and Xian-Ming Pan and Chu Wang and Yu Zhang and Guo-zhong Jing},
  journal={FEBS Letters},
Molecular cloning and characterization of a novel adenylate kinase 3 gene from Clonorchis sinensis
From a Clonorchis sinensis adult worm cDNA library, a cDNA clone is isolated encoding a novel AK3 isozyme that catalyzes reversible high energy phosphoryl transfer reactions between ATP and AMP to generate ADP.
Helical kink and channel behaviour: a comparative study with the peptaibols alamethicin, trichotoxin and antiamoebin
This short review compares the monomeric structures of three selected peptaibols that widely differ with regards to their near-central kink angles and dipolar moment orientations and shows these structural features to be correlated to the different patterns of channel activity, both at the macroscopic and single-channel levels of investigation.
Exploring the roles of substrate‐binding surface of the chaperone site in the chaperone activity of trigger factor
The results suggest that the folding‐favorable interface between the cradle and substrate was dynamic during TF‐assisted protein folding, which enabled TF to be involved in the folding of substrate in an aggressive manner rather than acting as a classic holdase.


Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding.
High-resolution crystallographic analysis of the proline-containing mutant protein (Ala-82----Pro) shows that its three-dimensional structure is essentially identical with the wild- type enzyme, and the overall structure of the other mutant enzyme is also very similar to wild-type lysozyme, although there are localized conformational adjustments in the vicinity of the altered amino acid.
Proline isomerism leads to multiple folded conformations of calbindin D9k: direct evidence from two-dimensional 1H NMR spectroscopy.
Analysis of short proton-proton distances along the peptide backbone, identified by two-dimensional nuclear Overhauser effect spectroscopy, provides unambiguous evidence that the two forms of the folded protein differ only in the isomerization state of the peptides between Gly-42 and Pro-43.
High level expression of chicken muscle adenylate kinase in Escherichia coli.
Chicken muscle adenylate kinase was produced in a large amount in Escherichia coli cells harboring an expression plasmid, pKK-cAKl-1, andMichaelis constants for ATP, ADP, and AMP of the enzyme thus synthesized were essentially identical to those determined with the enzyme in crude extracts of chicken skeletal muscle.
Proline motifs in peptides and their biological processing
The conformational restrictions imposed by proline motifs in a peptide chain appear to imply important structural or biological functions as can be deduced from their often remarkably high degree of conservation as found in many proteins and peptides.
Stabilization of alpha-helical structures in short peptides via end capping.
The alpha-helix-stabilizing effect of different amino acid residues at the helical termini of short peptides in aqueous solution has been determined and results agree with the Presta and Rose hypothesis.