Conformation-selective methylation of geminivirus DNA.

  title={Conformation-selective methylation of geminivirus DNA.},
  author={Tobias Paprotka and Kathrin Deuschle and Verena Metzler and Holger Jeske},
  journal={Journal of virology},
  volume={85 22},
Geminiviruses with small circular single-stranded DNA genomes replicate in plant cell nuclei by using various double-stranded DNA (dsDNA) intermediates: distinct open circular and covalently closed circular as well as heterogeneous linear DNA. Their DNA may be methylated partially at cytosine residues, as detected previously by bisulfite sequencing and subsequent PCR. In order to determine the methylation patterns of the circular molecules, the DNAs of tomato yellow leaf curl Sardinia virus… CONTINUE READING
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