Conformation of protein secreted across bacterial outer membranes: a study of enterotoxin translocation from Vibrio cholerae.

@article{Hirst1987ConformationOP,
  title={Conformation of protein secreted across bacterial outer membranes: a study of enterotoxin translocation from Vibrio cholerae.},
  author={Timothy R. Hirst and Jan Holmgren},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1987},
  volume={84 21},
  pages={7418-22}
}
The secretion of enterotoxin by Vibrio cholerae is punctuated by the transient entry of the toxin subunits into the periplasm. In this paper, we show that the subunits oligomerize into an assembled holotoxin within the periplasm prior to their secretion across the outer membrane. The rate of toxin assembly was studied by pulse-labeling cells with [35S]-methionine and then monitoring the turnover of radiolabeled subunits as they assembled within the periplasm. The subunits entered the periplasm… CONTINUE READING

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