Conformation dependence of a monoclonal antibody defined epitope on free human kappa chains.

@article{Raison1985ConformationDO,
  title={Conformation dependence of a monoclonal antibody defined epitope on free human kappa chains.},
  author={Robert L. Raison and Heather Boux},
  journal={Molecular immunology},
  year={1985},
  volume={22 12},
  pages={1393-8}
}
Chemically and enzymatically modified kappa chains were tested by inhibition radioimmunoassay for their ability to block the binding of antibody K-1-21 with native kappa chains. Complete reduction and carboxymethylation of intrachain disulphide bonds destroyed the free kappa-chain epitope, a result confirmed by Western blotting of unreduced and reduced kappa monomers and dimers. Purified V kappa fragments failed to block the homologous interaction while inhibition was obtained with a pepsin… CONTINUE READING

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