Conformation change in reconstituted thin filament studied with fluorescence energy transfer between epsilon-ADP bound to F-actin and NBD-Cl bound to troponin-C.

  • Marie Miki
  • Published 1979 in Biochimica et biophysica acta

Abstract

The interaction of troponin and F-actin was studied by the methods of fluorescence energy transfer. We used epsilon-ADP bound to F-actin as a fluorescence donor and 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole bound to troponin-C in troponin as a fluorescence acceptor. The efficiency of the fluorescence energy transfer of this system was larger in the absence of… (More)

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Cite this paper

@article{Miki1979ConformationCI, title={Conformation change in reconstituted thin filament studied with fluorescence energy transfer between epsilon-ADP bound to F-actin and NBD-Cl bound to troponin-C.}, author={Marie Miki}, journal={Biochimica et biophysica acta}, year={1979}, volume={578 1}, pages={96-106} }